Review





Similar Products

anti neurod1  (Bio-Techne corporation)
99
Bio-Techne corporation anti neurod1
( A ) Schematic of the protocol for COs’ maturation using platelet-derived growth factor AA and insulin-like growth factor 1. ( B – E ) Whole-mount immunolabeling of D65 WT COs containing differentiated <t>NEUROD1</t> + neurons ( B ), GFAP + neural precursor cells and GFAP-AQP4 + astrocytes ( C and D ), and OLIG2 + oligodendrocyte precursor cells and O4 + premyelinating oligodendrocytes ( E ); in blue is the nuclear staining with DAPI. B : scale bar 75 μm; C : scale bar 75 μm; inset in C : scale bar 25 μm; D : scale bar 40 μm; E : scale bar 75 μm; inset in E : scale bar 40 μm. Arrows in D indicate AQP4 staining in the membrane of GFAP + cells. The arrow in the inset in E points to the cellular body of a representative oligodendrocyte; arrowheads point to its cellular processes. ( F and G ) Volcano plots showing the distribution of DEGs in WT vs. Mut1 ( F ) and in WT vs. Mut2 ( G ); each point represents the average of 3 (WT) or 2 (MCT8-deficient) samples consisting of 4 pooled COs for each transcript. ( H ) Venn comparison of WT vs. Mut1, WT vs. Mut2, and Mut1 vs. Mut2 DEGs. A common cluster (cluster E) between WT vs. Mut1 and WT vs. Mut2 containing 949 DEGs was identified. ( I ) Venn comparison of the 949 DEGs in cluster E shows similar transcriptomic changes in both MCT8-deficient COs. ( J ) Heatmap depicting the top 63 DEGs from cluster E. ( K ) KEGG pathway analysis of the top 63 DEGs in J . ( L – O ) Heatmaps of T3-regulated genes involved in cerebral cortex development ( L ), neural cell migration ( M ), astrocytes and myelination ( N ), and neurotransmitter receptors, transcription factors, potassium channels, and extracellular matrix protein ( O ). DEG thresholds (FDR ≤ 0.25 and fold-change ± 4) were identified by gene set–specific analysis in the Partek Flow platform.
Anti Neurod1, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+neurod1/Human%2FMouse+NeuroD1+Antibody/pmc11128209-189-57-59
Average 99 stars, based on 1 article reviews
anti neurod1 - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
goat anti neurod1  (R&D Systems)
93
R&D Systems goat anti neurod1
( A ) Schematic of the protocol for COs’ maturation using platelet-derived growth factor AA and insulin-like growth factor 1. ( B – E ) Whole-mount immunolabeling of D65 WT COs containing differentiated <t>NEUROD1</t> + neurons ( B ), GFAP + neural precursor cells and GFAP-AQP4 + astrocytes ( C and D ), and OLIG2 + oligodendrocyte precursor cells and O4 + premyelinating oligodendrocytes ( E ); in blue is the nuclear staining with DAPI. B : scale bar 75 μm; C : scale bar 75 μm; inset in C : scale bar 25 μm; D : scale bar 40 μm; E : scale bar 75 μm; inset in E : scale bar 40 μm. Arrows in D indicate AQP4 staining in the membrane of GFAP + cells. The arrow in the inset in E points to the cellular body of a representative oligodendrocyte; arrowheads point to its cellular processes. ( F and G ) Volcano plots showing the distribution of DEGs in WT vs. Mut1 ( F ) and in WT vs. Mut2 ( G ); each point represents the average of 3 (WT) or 2 (MCT8-deficient) samples consisting of 4 pooled COs for each transcript. ( H ) Venn comparison of WT vs. Mut1, WT vs. Mut2, and Mut1 vs. Mut2 DEGs. A common cluster (cluster E) between WT vs. Mut1 and WT vs. Mut2 containing 949 DEGs was identified. ( I ) Venn comparison of the 949 DEGs in cluster E shows similar transcriptomic changes in both MCT8-deficient COs. ( J ) Heatmap depicting the top 63 DEGs from cluster E. ( K ) KEGG pathway analysis of the top 63 DEGs in J . ( L – O ) Heatmaps of T3-regulated genes involved in cerebral cortex development ( L ), neural cell migration ( M ), astrocytes and myelination ( N ), and neurotransmitter receptors, transcription factors, potassium channels, and extracellular matrix protein ( O ). DEG thresholds (FDR ≤ 0.25 and fold-change ± 4) were identified by gene set–specific analysis in the Partek Flow platform.
Goat Anti Neurod1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+neurod1/Human%2FMouse+NeuroD1+Antibody/pm41172953-95-54-57
Average 93 stars, based on 1 article reviews
goat anti neurod1 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
anti goat neurod1  (R&D Systems)
93
R&D Systems anti goat neurod1
( A ) Schematic of the protocol for COs’ maturation using platelet-derived growth factor AA and insulin-like growth factor 1. ( B – E ) Whole-mount immunolabeling of D65 WT COs containing differentiated <t>NEUROD1</t> + neurons ( B ), GFAP + neural precursor cells and GFAP-AQP4 + astrocytes ( C and D ), and OLIG2 + oligodendrocyte precursor cells and O4 + premyelinating oligodendrocytes ( E ); in blue is the nuclear staining with DAPI. B : scale bar 75 μm; C : scale bar 75 μm; inset in C : scale bar 25 μm; D : scale bar 40 μm; E : scale bar 75 μm; inset in E : scale bar 40 μm. Arrows in D indicate AQP4 staining in the membrane of GFAP + cells. The arrow in the inset in E points to the cellular body of a representative oligodendrocyte; arrowheads point to its cellular processes. ( F and G ) Volcano plots showing the distribution of DEGs in WT vs. Mut1 ( F ) and in WT vs. Mut2 ( G ); each point represents the average of 3 (WT) or 2 (MCT8-deficient) samples consisting of 4 pooled COs for each transcript. ( H ) Venn comparison of WT vs. Mut1, WT vs. Mut2, and Mut1 vs. Mut2 DEGs. A common cluster (cluster E) between WT vs. Mut1 and WT vs. Mut2 containing 949 DEGs was identified. ( I ) Venn comparison of the 949 DEGs in cluster E shows similar transcriptomic changes in both MCT8-deficient COs. ( J ) Heatmap depicting the top 63 DEGs from cluster E. ( K ) KEGG pathway analysis of the top 63 DEGs in J . ( L – O ) Heatmaps of T3-regulated genes involved in cerebral cortex development ( L ), neural cell migration ( M ), astrocytes and myelination ( N ), and neurotransmitter receptors, transcription factors, potassium channels, and extracellular matrix protein ( O ). DEG thresholds (FDR ≤ 0.25 and fold-change ± 4) were identified by gene set–specific analysis in the Partek Flow platform.
Anti Goat Neurod1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+neurod1/Human%2FMouse+NeuroD1+Antibody/pm40963028-1330-21-23
Average 93 stars, based on 1 article reviews
anti goat neurod1 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
anti goat neurod1 antibody  (R&D Systems)
93
R&D Systems anti goat neurod1 antibody
( A ) Schematic of the protocol for COs’ maturation using platelet-derived growth factor AA and insulin-like growth factor 1. ( B – E ) Whole-mount immunolabeling of D65 WT COs containing differentiated <t>NEUROD1</t> + neurons ( B ), GFAP + neural precursor cells and GFAP-AQP4 + astrocytes ( C and D ), and OLIG2 + oligodendrocyte precursor cells and O4 + premyelinating oligodendrocytes ( E ); in blue is the nuclear staining with DAPI. B : scale bar 75 μm; C : scale bar 75 μm; inset in C : scale bar 25 μm; D : scale bar 40 μm; E : scale bar 75 μm; inset in E : scale bar 40 μm. Arrows in D indicate AQP4 staining in the membrane of GFAP + cells. The arrow in the inset in E points to the cellular body of a representative oligodendrocyte; arrowheads point to its cellular processes. ( F and G ) Volcano plots showing the distribution of DEGs in WT vs. Mut1 ( F ) and in WT vs. Mut2 ( G ); each point represents the average of 3 (WT) or 2 (MCT8-deficient) samples consisting of 4 pooled COs for each transcript. ( H ) Venn comparison of WT vs. Mut1, WT vs. Mut2, and Mut1 vs. Mut2 DEGs. A common cluster (cluster E) between WT vs. Mut1 and WT vs. Mut2 containing 949 DEGs was identified. ( I ) Venn comparison of the 949 DEGs in cluster E shows similar transcriptomic changes in both MCT8-deficient COs. ( J ) Heatmap depicting the top 63 DEGs from cluster E. ( K ) KEGG pathway analysis of the top 63 DEGs in J . ( L – O ) Heatmaps of T3-regulated genes involved in cerebral cortex development ( L ), neural cell migration ( M ), astrocytes and myelination ( N ), and neurotransmitter receptors, transcription factors, potassium channels, and extracellular matrix protein ( O ). DEG thresholds (FDR ≤ 0.25 and fold-change ± 4) were identified by gene set–specific analysis in the Partek Flow platform.
Anti Goat Neurod1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+neurod1/Human%2FMouse+NeuroD1+Antibody/pm40963028-1339-1-8
Average 93 stars, based on 1 article reviews
anti goat neurod1 antibody - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


( A ) Schematic of the protocol for COs’ maturation using platelet-derived growth factor AA and insulin-like growth factor 1. ( B – E ) Whole-mount immunolabeling of D65 WT COs containing differentiated NEUROD1 + neurons ( B ), GFAP + neural precursor cells and GFAP-AQP4 + astrocytes ( C and D ), and OLIG2 + oligodendrocyte precursor cells and O4 + premyelinating oligodendrocytes ( E ); in blue is the nuclear staining with DAPI. B : scale bar 75 μm; C : scale bar 75 μm; inset in C : scale bar 25 μm; D : scale bar 40 μm; E : scale bar 75 μm; inset in E : scale bar 40 μm. Arrows in D indicate AQP4 staining in the membrane of GFAP + cells. The arrow in the inset in E points to the cellular body of a representative oligodendrocyte; arrowheads point to its cellular processes. ( F and G ) Volcano plots showing the distribution of DEGs in WT vs. Mut1 ( F ) and in WT vs. Mut2 ( G ); each point represents the average of 3 (WT) or 2 (MCT8-deficient) samples consisting of 4 pooled COs for each transcript. ( H ) Venn comparison of WT vs. Mut1, WT vs. Mut2, and Mut1 vs. Mut2 DEGs. A common cluster (cluster E) between WT vs. Mut1 and WT vs. Mut2 containing 949 DEGs was identified. ( I ) Venn comparison of the 949 DEGs in cluster E shows similar transcriptomic changes in both MCT8-deficient COs. ( J ) Heatmap depicting the top 63 DEGs from cluster E. ( K ) KEGG pathway analysis of the top 63 DEGs in J . ( L – O ) Heatmaps of T3-regulated genes involved in cerebral cortex development ( L ), neural cell migration ( M ), astrocytes and myelination ( N ), and neurotransmitter receptors, transcription factors, potassium channels, and extracellular matrix protein ( O ). DEG thresholds (FDR ≤ 0.25 and fold-change ± 4) were identified by gene set–specific analysis in the Partek Flow platform.

Journal: JCI Insight

Article Title: Impaired T3 uptake and action in MCT8-deficient cerebral organoids underlie Allan-Herndon-Dudley syndrome

doi: 10.1172/jci.insight.174645

Figure Lengend Snippet: ( A ) Schematic of the protocol for COs’ maturation using platelet-derived growth factor AA and insulin-like growth factor 1. ( B – E ) Whole-mount immunolabeling of D65 WT COs containing differentiated NEUROD1 + neurons ( B ), GFAP + neural precursor cells and GFAP-AQP4 + astrocytes ( C and D ), and OLIG2 + oligodendrocyte precursor cells and O4 + premyelinating oligodendrocytes ( E ); in blue is the nuclear staining with DAPI. B : scale bar 75 μm; C : scale bar 75 μm; inset in C : scale bar 25 μm; D : scale bar 40 μm; E : scale bar 75 μm; inset in E : scale bar 40 μm. Arrows in D indicate AQP4 staining in the membrane of GFAP + cells. The arrow in the inset in E points to the cellular body of a representative oligodendrocyte; arrowheads point to its cellular processes. ( F and G ) Volcano plots showing the distribution of DEGs in WT vs. Mut1 ( F ) and in WT vs. Mut2 ( G ); each point represents the average of 3 (WT) or 2 (MCT8-deficient) samples consisting of 4 pooled COs for each transcript. ( H ) Venn comparison of WT vs. Mut1, WT vs. Mut2, and Mut1 vs. Mut2 DEGs. A common cluster (cluster E) between WT vs. Mut1 and WT vs. Mut2 containing 949 DEGs was identified. ( I ) Venn comparison of the 949 DEGs in cluster E shows similar transcriptomic changes in both MCT8-deficient COs. ( J ) Heatmap depicting the top 63 DEGs from cluster E. ( K ) KEGG pathway analysis of the top 63 DEGs in J . ( L – O ) Heatmaps of T3-regulated genes involved in cerebral cortex development ( L ), neural cell migration ( M ), astrocytes and myelination ( N ), and neurotransmitter receptors, transcription factors, potassium channels, and extracellular matrix protein ( O ). DEG thresholds (FDR ≤ 0.25 and fold-change ± 4) were identified by gene set–specific analysis in the Partek Flow platform.

Article Snippet: Immunofluorescence studies were performed as previously described , and COs were incubated with primary and secondary antibodies using the following dilutions: mouse monoclonal anti-Tuj1 1:1,000 (Bio-Techne; AF4320), anti-Sox2 1:1,000 (Abcam; 109186), anti-Ki67 1:1,000 (MilliporeSigma; MAB4190), anti-PVIM 1:400 (MBL; D076-3), anti-PH3 1:400 (Cell Signaling Technology; 9701S), anti-SATB2 1:400 (Abcam; ab92446), anti-Vimentin (Novus; NB300-223), anti-MCT8 antibody 1:400 (Atlas; HPA003353), anti-NEUROD1 1:400 (Bio-Techne; AF2746), anti-GFAP 1:200 (Novus; 05198), anti-AQP4 1:200 (Novus; 52872), anti-Olig2 (Abcam; ab109186), anti-O4 (Bio-Techne; MAB1326), Alexa Fluor 488–conjugated goat anti-mouse IgG 1:200 (Vector Laboratories; DK-2488), Alexa Fluor 594–conjugated horse anti-rabbit IgG 1:200 (Vector Laboratories; DK-1594), and Alexa Fluor 488–conjugated horse anti-rabbit IgG 1:200 (Vector Laboratories; DI-1788).

Techniques: Derivative Assay, Immunolabeling, Staining, Membrane, Comparison, Migration